Foundations In Microbiology 6th Edition By -Kathleen Park Talaro -Test Bank
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Sample
Test
3
Student:
___________________________________________________________________________
1. The
Five I’s of studying microorganisms include all of the following except
A. inoculation.
B. incubation.
C. infection.
D. isolation.
E. identification.
2. All
of the following are examples of different types of microbiological media except
A. broth.
B. enriched.
C. agar.
D. petri dish.
E. gelatin.
3. The
term that refers to the purposeful addition of microorganisms into a laboratory
nutrient medium is
A. isolation.
B. inoculation.
C. immunization.
D. infection.
E. contamination.
4. Which
of the following is essential for development of discrete, isolated colonies?
A. broth medium
B. differential medium
C. selective medium
D. solid medium
E. assay medium
5. A
pure culture contains only
A. one species of microorganism.
B. bacteria.
C. a variety of microbes from one source.
D. a variety of species from the same genus.
E. None of the choices are correct.
6. Which
of the following will result when 1% to 5% agar is added to nutrient broth,
boiled and cooled?
A. a pure culture
B. a mixed culture
C. a solid medium
D. a liquid medium
E. a contaminated medium
7. A
microbiologist inoculates Staphylococcus aureus into a
culture medium. Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are
determined to be growing in this culture. What is the most likely explanation?
A. The microbiologist used too much inoculum.
B. The culture is contaminated.
C. The incubation temperature was incorrect.
D. The culture medium must be selective.
E. The culture medium must be differential.
8. A
microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture
medium. Following incubation, only the E. coli grows in the
culture. What is the most likely explanation?
A. The microbiologist used too much inoculum.
B. The culture is contaminated.
C. The incubation temperature was incorrect.
D. The culture medium must be selective.
E. The culture medium must be differential.
9. Which
method often results in colonies developing down throughout the agar and some
colonies on the surface?
A. streak plate.
B. spread plate.
C. pour plate.
D. All of the choices are correct.
E. None of the choices are correct.
10. A
common medium used for growing fastidious bacteria is
A. blood agar.
B. trypticase soy agar.
C. mannitol salt agar.
D. MacConkey medium.
E. a reducing medium.
11. A
nutrient medium that has all of its chemical components identified and their
precise concentrations known and reproducible, would be termed
A. complex.
B. reducing.
C. enriched.
D. enumeration.
E. synthetic.
12. A
reducing medium contains
A. sugars that can be fermented.
B. extra oxygen.
C. hemoglobin, vitamins or other growth factors.
D. substances that remove oxygen.
E. inhibiting agents.
13. Which
type of medium is able to distinguish different species or types of
microorganisms based on an observable change in the colonies or in the medium?
A. differential
B. selective
C. enumeration
D. enriched
E. reducing
14. A
microbiologist decides to use a nutrient medium that contains thioglycollic
acid. What type of microbe is she attempting to culture?
A. fastidious
B. gram positive
C. anaerobe
D. gram negative
E. virus
15. For
which bacterial genus is mannitol salt agar selective?
A. Salmonella
B. Streptococcus
C. Neisseria
D. Staphylococcus
E. Escherichia
16. A
microbiologist must culture a patient’s feces for intestinal pathogens. Which
of the following would likely be present in selective media for analyzing this
fecal specimen?
A. NaCl
B. sheep red blood cells
C. bile salts
D. thioglycollic acid
E. peptone
17. Which
of the following characteristics refers to the microscope’s ability to show two
separate entities as separate and distinct?
A. resolving power
B. magnification
C. refraction
D. All of the choices are correct.
E. None of the choices are correct.
18. Which
of the following magnifies the specimen to produce the real image of the specimen?
A. condenser
B. objective lens
C. ocular lens
D. body
E. nosepiece
19. If a
microbiologist is studying a specimen at a total magnification of 950X, what is
the magnifying power of the objective lens if the ocular lens is 10X?
A. 100X
B. 950X
C. 85X
D. 850X
E. 95X
20. All
of the following are diameters of cells that would be resolved in a microscope
with a limit of resolution of 0.2µm except
A. 0.2 µm.
B. 0.2 mm.
C. 0.1 µm.
D. 0.3 µm.
E. 2.0 µm.
21. The
wavelength of light used plus the numerical aperature governs
A. illumination.
B. resolution.
C. magnification.
D. size of the field.
E. All of the choices are correct.
22. The
type of microscope in which you would see brightly illuminated specimens
against a black background is
A. bright-field.
B. dark-field.
C. phase-contrast.
D. fluorescence.
E. electron.
23. This
microscope does not use
light in forming the specimen image:
A. bright-field
B. dark-field
C. phase-contrast
D. fluorescence
E. electron
24. This
microscope achieves the greatest resolution and highest magnification:
A. bright-field
B. dark-field
C. phase-contrast
D. fluorescence
E. electron
25. This
microscope shows cells against a bright background and also shows intracellular
structures of unstained cells based on their varying densities:
A. bright-field
B. dark-field
C. phase-contrast
D. fluorescence
E. electron
26. This
microscope is the most widely used and shows cells against a bright background:
A. bright-field
B. dark-field
C. phase-contrast
D. fluorescence
E. electron
27. All
of the following pertain to the fluorescence microscope except it
A. uses electron’s to produce a specimen image.
B. type of compound microscope.
C. requires the use of dyes like acridine and fluorescein.
D. commonly used to diagnose certain infections.
E. requires an ultraviolet radiation source.
28. Which
is incorrect about chocolate agar?
A. It can be used to cultivate Neisseria.
B. It usually uses sheep blood that has been heated.
C. It has chocolate extract in it.
D. It is an enriched medium.
E. It is used to grow fastidious bacteria.
29. Which
microscope bombards a whole, metal-coated specimen with electrons moving back
and forth over it?
A. fluorescence
B. differential interference contrast
C. scanning electron
D. transmission electron
E. phase-contrast
30. The
specimen preparation that is best for viewing cell motility is
A. hanging drop.
B. fixed stained smear.
C. gram stain.
D. negative stain.
E. flagellar stain.
31. The
primary purpose of staining cells on a microscope slide is to
A. kill them.
B. secure them to the slide.
C. enlarge the cells.
D. add contrast in order to see them better.
E. see motility.
32. The
Gram stain, acid-fast stain and endospore stain have the following in common:
A. used on a wet mount of the specimen
B. use heat to force the dye into cell structures
C. outcome based on cell wall differences
D. use a negative stain technique
E. are differential stains
33. Basic
dyes are
A. attracted to the acidic substances of bacterial cells.
B. anionic.
C. used in negative staining.
D. repelled by cells.
E. dyes such as India ink and nigrosin.
34. A
microbiologist makes a fixed smear of bacterial cells and stains them with
Loeffler’s methylene blue. All the cells appear blue under the oil lens. This
is an example of
A. negative staining.
B. using an acidic dye.
C. simple staining.
D. using the acid-fast stain.
E. capsule staining.
35. Media
that contains extracts from plants, animals or yeasts are
A. synthetic.
B. complex.
C. reducing.
D. enriched.
E. All of the choices are correct.
36. Brain-heart
infusion, trypticase soy agar (TSA) and nutrient agar are all examples of which
type of media?
A. synthetic
B. reducing
C. enriched
D. nonsynthetic
E. selective
37. Bacteria
that require special growth factors and complex organic substances are called
A. fastidious.
B. pathogenic.
C. harmless.
D. anaerobic.
E. aerobic.
38. A
media is designed that allows only Staphylococci to grow. In addition, S. aureus colonies
have a yellow halo around them and other staphylococci appear white. This type
of media is
A. selective only.
B. differential only.
C. both selective and differential.
D. a reducing media.
E. enriched.
39. All
of the following are examples of basic dyes except
A. methylene blue.
B. nigrosin.
C. crystal violet.
D. safranin.
E. fuchsin.
40. Which
type of media can be used to determine if a bacteria is motile?
A. SIM
B. MacConkey
C. Enriched media
D. Thayer-Martin media
E. Chocolate agar
41. All
of the following are correct about agar except
A. it is flexible.
B. it melts at the boiling point of water (100oC).
C. it is a source of nutrition for bacteria.
D. it solidifies below 42oC.
E. it is solid at room temperature.
42. Which
of the following media is useful for cultivating fungi?
A. Sabouraud’s agar
B. MacConkey agar
C. Tomato juice agar
D. Phenylethanol agar
E. Mueller tellurite
43. Which
of the following puts the Five “I”s in their correct order?
A. inoculation, incubation, isolation, inspection, identification
B. isolation, inspection, inoculation, incubation, identification
C. incubation, inspection, isolation, identification, inoculation
D. inspection, identification, isolation, incubation, inoculation
E. inspection, isolation, incubation, inoculation, identification
44. Why
is oil of immersion often used when viewing specimens under the microscope?
A. to stain the cells blue
B. to prevent the smear from drying out
C. to slow bacterial movement so you can see cells better
D. to increase the resolution
E. to reduce the amount of heat reaching the slide from the light source
45. The
procedures for culturing a microorganism requires the use of a microscope.
True False
46. One
colony typically develops from the growth of several parent bacterial cells.
True False
47. Some
microbes are not capable of growing on artificial media.
True False
48. Mixed
cultures are also referred to as contaminated cultures.
True False
49. A
medium that is gel-like has less agar in it compared to a solid medium.
True False
50. A
selective medium contains one or more substances that inhibit growth of certain
microbes in order to facilitate the growth of other microbes.
True False
51. A
bacterial species that grows on blood agar but will not grow on trypticase soy
agar is termed an anaerobe.
True False
52. Fixed
smears of specimens are required in order to perform the Gram stain and
endospore stain on the specimens.
True False
53. The
bending of light rays as they pass form one medium to another is called
refraction.
True False
54. At
the end of the Gram stain, gram positive bacteria will be seen as purple cells.
True False
55. Scanning
tunneling and atomic force microscopes are used to image the detailed structure
of biological molecules.
True False
56. The
correct microbiological term for the tiny sample of specimen that is put into a
nutrient medium in order to produce a culture is the _____.
________________________________________
57. The
three physical forms of laboratory media are: solid, semisolid, and _____.
________________________________________
58. Bacteria
that require special growth factors and complex nutrients are termed _____.
________________________________________
59. _____
is the term for a culture made from one isolated colony.
________________________________________
60. Newly
inoculated cultures must be _____ at a specific temperature and time to
encourage growth.
________________________________________
61. Magnification
is achieved in a compound microscope through the initial magnification of the
specimen by the _____ lens. This image is then projected to the _____ lens that
will further magnify the specimen to form a virtual image received by the eye.
________________________________________
62. The
_____ of the microscope holds and allows selection of the objective lenses.
________________________________________
63. _____
dyes have a negative charge on the chromophore and are repelled by bacterial
cells.
________________________________________
64. _____
has the same optical qualities as glass and thus prevents refractive loss of
light as it passes from the slide to the objective lens.
________________________________________
65. Compare
and contrast the reagents and functions of negative staining versus positive
staining.
66. Explain
the difference and significance between a contaminated culture and a mixed
culture.
67. A
contaminated food sample contains several different species of bacteria. A food
microbiologist is interested in studying just one of these species. Describe
the sequence of procedures that the microbiologist must perform in order to
obtain a pure culture of the bacterial species of interest from this food
sample. Detail all the necessary media and equipment.
68. Explain
how and why immersion oil increases resolution but not magnification when using
the 100X objective.
3 Key
1. The
Five I’s of studying microorganisms include all of the following except
A. inoculation.
B. incubation.
C. infection.
D. isolation.
E. identification.
Talaro – 003 Chapter… #1
2. All
of the following are examples of different types of microbiological media except
A. broth.
B. enriched.
C. agar.
D. petri
dish.
E. gelatin.
Talaro – 003 Chapter… #2
3. The
term that refers to the purposeful addition of microorganisms into a laboratory
nutrient medium is
A.isolation.
B. inoculation.
C. immunization.
D. infection.
E. contamination.
Talaro – 003 Chapter… #3
4. Which
of the following is essential for development of discrete, isolated colonies?
A.broth medium
B. differential medium
C. selective medium
D. solid
medium
E. assay medium
Talaro – 003 Chapter… #4
5. A
pure culture contains only
A.one
species of microorganism.
B. bacteria.
C. a variety of microbes from one source.
D. a variety of species from the same genus.
E. None of the choices are correct.
Talaro – 003 Chapter… #5
6. Which
of the following will result when 1% to 5% agar is added to nutrient broth,
boiled and cooled?
A.a pure culture
B. a mixed culture
C. a
solid medium
D. a liquid medium
E. a contaminated medium
Talaro – 003 Chapter… #6
7. A
microbiologist inoculates Staphylococcus aureus into a
culture medium. Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are
determined to be growing in this culture. What is the most likely explanation?
A.The microbiologist used too much inoculum.
B. The
culture is contaminated.
C. The incubation temperature was incorrect.
D. The culture medium must be selective.
E. The culture medium must be differential.
Talaro – 003 Chapter… #7
8. A
microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture
medium. Following incubation, only the E. coli grows in the
culture. What is the most likely explanation?
A.The microbiologist used too much inoculum.
B. The culture is contaminated.
C. The incubation temperature was incorrect.
D. The
culture medium must be selective.
E. The culture medium must be differential.
Talaro – 003 Chapter… #8
9. Which
method often results in colonies developing down throughout the agar and some
colonies on the surface?
A.streak plate.
B. spread plate.
C. pour
plate.
D. All of the choices are correct.
E. None of the choices are correct.
Talaro – 003 Chapter… #9
10. A
common medium used for growing fastidious bacteria is
A.blood
agar.
B. trypticase soy agar.
C. mannitol salt agar.
D. MacConkey medium.
E. a reducing medium.
Talaro – 003 Chapter… #10
11. A
nutrient medium that has all of its chemical components identified and their
precise concentrations known and reproducible, would be termed
A.complex.
B. reducing.
C. enriched.
D. enumeration.
E. synthetic.
Talaro – 003 Chapter… #11
12. A
reducing medium contains
A.sugars that can be fermented.
B. extra oxygen.
C. hemoglobin, vitamins or other growth factors.
D. substances
that remove oxygen.
E. inhibiting agents.
Talaro – 003 Chapter… #12
13. Which
type of medium is able to distinguish different species or types of
microorganisms based on an observable change in the colonies or in the medium?
A.differential
B. selective
C. enumeration
D. enriched
E. reducing
Talaro – 003 Chapter… #13
14. A microbiologist
decides to use a nutrient medium that contains thioglycollic acid. What type of
microbe is she attempting to culture?
A.fastidious
B. gram positive
C. anaerobe
D. gram negative
E. virus
Talaro – 003 Chapter… #14
15. For
which bacterial genus is mannitol salt agar selective?
A.Salmonella
B. Streptococcus
C. Neisseria
D. Staphylococcus
E. Escherichia
Talaro – 003 Chapter… #15
16. A
microbiologist must culture a patient’s feces for intestinal pathogens. Which
of the following would likely be present in selective media for analyzing this
fecal specimen?
A.NaCl
B. sheep red blood cells
C. bile
salts
D. thioglycollic acid
E. peptone
Talaro – 003 Chapter… #16
17. Which
of the following characteristics refers to the microscope’s ability to show two
separate entities as separate and distinct?
A.resolving
power
B. magnification
C. refraction
D. All of the choices are correct.
E. None of the choices are correct.
Talaro – 003 Chapter… #17
18. Which
of the following magnifies the specimen to produce the real image of the
specimen?
A.condenser
B. objective
lens
C. ocular lens
D. body
E. nosepiece
Talaro – 003 Chapter… #18
19. If a
microbiologist is studying a specimen at a total magnification of 950X, what is
the magnifying power of the objective lens if the ocular lens is 10X?
A.100X
B. 950X
C. 85X
D. 850X
E. 95X
Talaro – 003 Chapter… #19
20. All
of the following are diameters of cells that would be resolved in a microscope
with a limit of resolution of 0.2µm except
A. 0.2 µm.
B. 0.2 mm.
C. 0.1
µm.
D. 0.3 µm.
E. 2.0 µm.
Talaro – 003 Chapter… #20
21. The
wavelength of light used plus the numerical aperature governs
A.illumination.
B. resolution.
C. magnification.
D. size of the field.
E. All of the choices are correct.
Talaro – 003 Chapter… #21
22. The
type of microscope in which you would see brightly illuminated specimens
against a black background is
A.bright-field.
B. dark-field.
C. phase-contrast.
D. fluorescence.
E. electron.
Talaro – 003 Chapter… #22
23. This
microscope does not use
light in forming the specimen image:
A.bright-field
B. dark-field
C. phase-contrast
D. fluorescence
E. electron
Talaro – 003 Chapter… #23
24. This
microscope achieves the greatest resolution and highest magnification:
A.bright-field
B. dark-field
C. phase-contrast
D. fluorescence
E. electron
Talaro – 003 Chapter… #24
25. This
microscope shows cells against a bright background and also shows intracellular
structures of unstained cells based on their varying densities:
A.bright-field
B. dark-field
C. phase-contrast
D. fluorescence
E. electron
Talaro – 003 Chapter… #25
26. This
microscope is the most widely used and shows cells against a bright background:
A.bright-field
B. dark-field
C. phase-contrast
D. fluorescence
E. electron
Talaro – 003 Chapter… #26
27. All
of the following pertain to the fluorescence microscope except it
A.uses
electron’s to produce a specimen image.
B. type of compound microscope.
C. requires the use of dyes like acridine and fluorescein.
D. commonly used to diagnose certain infections.
E. requires an ultraviolet radiation source.
Talaro – 003 Chapter… #27
28. Which
is incorrect about chocolate agar?
A.It can be used to cultivate Neisseria.
B. It usually uses sheep blood that has been heated.
C. It
has chocolate extract in it.
D. It is an enriched medium.
E. It is used to grow fastidious bacteria.
Talaro – 003 Chapter… #28
29. Which
microscope bombards a whole, metal-coated specimen with electrons moving back
and forth over it?
A.fluorescence
B. differential interference contrast
C. scanning
electron
D. transmission electron
E. phase-contrast
Talaro – 003 Chapter… #29
30. The
specimen preparation that is best for viewing cell motility is
A.hanging
drop.
B. fixed stained smear.
C. gram stain.
D. negative stain.
E. flagellar stain.
Talaro – 003 Chapter… #30
31. The
primary purpose of staining cells on a microscope slide is to
A.kill them.
B. secure them to the slide.
C. enlarge the cells.
D. add
contrast in order to see them better.
E. see motility.
Talaro – 003 Chapter… #31
32. The
Gram stain, acid-fast stain and endospore stain have the following in common:
A.used on a wet mount of the specimen
B. use heat to force the dye into cell structures
C. outcome based on cell wall differences
D. use a negative stain technique
E. are
differential stains
Talaro – 003 Chapter… #32
33. Basic
dyes are
A.attracted
to the acidic substances of bacterial cells.
B. anionic.
C. used in negative staining.
D. repelled by cells.
E. dyes such as India ink and nigrosin.
Talaro – 003 Chapter… #33
34. A
microbiologist makes a fixed smear of bacterial cells and stains them with
Loeffler’s methylene blue. All the cells appear blue under the oil lens. This
is an example of
A.negative staining.
B. using an acidic dye.
C. simple
staining.
D. using the acid-fast stain.
E. capsule staining.
Talaro – 003 Chapter… #34
35. Media
that contains extracts from plants, animals or yeasts are
A.synthetic.
B. complex.
C. reducing.
D. enriched.
E. All of the choices are correct.
Talaro – 003 Chapter… #35
36. Brain-heart
infusion, trypticase soy agar (TSA) and nutrient agar are all examples of which
type of media?
A.synthetic
B. reducing
C. enriched
D. nonsynthetic
E. selective
Talaro – 003 Chapter… #36
37. Bacteria
that require special growth factors and complex organic substances are called
A.fastidious.
B. pathogenic.
C. harmless.
D. anaerobic.
E. aerobic.
Talaro – 003 Chapter… #37
38. A
media is designed that allows only Staphylococci to grow. In addition, S. aureus colonies
have a yellow halo around them and other staphylococci appear white. This type
of media is
A.selective only.
B. differential only.
C. both
selective and differential.
D. a reducing media.
E. enriched.
Talaro – 003 Chapter… #38
39. All
of the following are examples of basic dyes except
A.methylene blue.
B. nigrosin.
C. crystal
violet.
D. safranin.
E. fuchsin.
Talaro – 003 Chapter… #39
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